5NQO
CU(I)-CSP3 (COPPER STORAGE PROTEIN 3) FROM METHYLOSINUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 44.901, 105.691, 45.625 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.620 - 1.150 |
| R-factor | 0.12582 |
| Rwork | 0.125 |
| R-free | 0.14542 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5arm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.625 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.620 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.052 | 0.516 |
| Number of reflections | 38830 | 1908 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM MgCl2, 100 mM Hepes pH 7.5, 30% PEG 400 |
