5NQF
Crystal structure of Plasmodium falciparum AMA1 in complex with a 39 aa PvRON2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2013-04-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91165 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.481, 38.248, 72.356 |
| Unit cell angles | 90.00, 98.55, 90.00 |
Refinement procedure
| Resolution | 35.780 - 1.900 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AMA1-FVO |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.920 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.083 | 0.569 |
| Number of reflections | 29741 | |
| <I/σ(I)> | 9.2 | 1.4 |
| Completeness [%] | 96.2 | 78.3 |
| Redundancy | 3 | 1.8 |
| CC(1/2) | 0.996 | 0.822 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 290 | PEG 3350, TRIS 8.2, SODIUM ACETATE, ISOPROPANOL, PH 8.2, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
