5NQ9
Crystal structure of laccases from Pycnoporus sanguineus, izoform II, monoclinic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.459, 61.962, 103.307 |
Unit cell angles | 90.00, 109.82, 90.00 |
Refinement procedure
Resolution | 48.650 - 2.720 |
R-factor | 0.1737 |
Rwork | 0.170 |
R-free | 0.24649 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xyb |
RMSD bond length | 0.016 |
RMSD bond angle | 1.857 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.880 |
High resolution limit [Å] | 2.720 | 2.720 |
Rmerge | 0.128 | 0.766 |
Number of reflections | 29229 | |
<I/σ(I)> | 10.38 | 1.85 |
Completeness [%] | 99.3 | 97.5 |
Redundancy | 3.71 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M magnesium formate dihydrate 20% PEG 3350 |