5NQ7
Crystal structure of laccases from Pycnoporus sanguineus, izoform I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 185.550, 185.550, 185.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.390 - 2.750 |
| R-factor | 0.16367 |
| Rwork | 0.161 |
| R-free | 0.20736 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xyb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.604 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.129 | 1.120 |
| Number of reflections | 28947 | 2870 |
| <I/σ(I)> | 27.7 | 3.78 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.8 | 26.8 |
| CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5 2.0 M ammonium sulfate |
