5NPY

Crystal structure of Helicobacter pylori flagellar hook protein FlgE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.8729
Spacegroup name	P 21 21 21
Unit cell lengths	43.209, 49.592, 238.261
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.551 - 2.292
R-factor	0.192
Rwork	0.188
R-free	0.26610
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.995
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	autoSHARP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.551	2.400
High resolution limit [Å]	2.290	2.290
Rmerge	0.148	0.671
Rpim	0.076	0.362
Number of reflections	23979	3106
<I/σ(I)>	9.7	2.3
Completeness [%]	98.5	90.8
Redundancy	5.5	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2 M Potassium Thiocyanate, 0.1 M Bis-Tris Propane pH 8.5, 20% w/v PEG3350
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2 M Potassium Thiocyanate, 0.1 M Bis-Tris Propane pH 8.5, 20% w/v PEG3350