5NPT
Structure of the N-terminal domain of the yeast telomerase reverse transcriptase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97858 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 38.195, 64.098, 126.064 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.030 - 2.400 |
R-factor | 0.18806 |
Rwork | 0.186 |
R-free | 0.22364 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | INCOMPLETE MODEL OF SE-MET VARIANT SOLVED BY SAD METHOD USING CRANK2 SOFTWARE |
RMSD bond length | 0.015 |
RMSD bond angle | 1.690 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.030 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.047 | 0.347 |
Rpim | 0.029 | 0.194 |
Number of reflections | 6202 | 3177 |
<I/σ(I)> | 20.6 | |
Completeness [%] | 98.0 | 98.9 |
Redundancy | 5 | 4.7 |
CC(1/2) | 0.998 | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 10.5 | 293 | Sodium-potassium-phosphate, sodium citrate and CAPS buffer |