5NPI
Structure of the Hepatitis C virus strain J4 glycoprotein E2 antigenic region 532-540 bound to the single chain variable fragment of the non-neutralizing antibody DAO5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98011 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 155.936, 155.936, 61.508 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.160 - 2.000 |
R-factor | 0.1977 |
Rwork | 0.197 |
R-free | 0.21660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.310 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 50405 | 6158 |
<I/σ(I)> | 10.3 | 1.9 |
Completeness [%] | 97.6 | 83.5 |
Redundancy | 5.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM MES, 28% PEG 400, 100mM Sodium acetate |