5NP0
Closed dimer of human ATM (Ataxia telangiectasia mutated)
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.7 |
| Number of Particle | 371671 |
| Software | Gautomatch,PHENIX,RELION,UCSFImage,Gctf,Coot |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
