5NOM

The structure of the G. violaceus guanidine II riboswitch P1 stem-loop with guanidine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9201
Spacegroup name	H 3 2
Unit cell lengths	55.700, 55.700, 132.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.193 - 1.930
R-factor	0.1988
Rwork	0.197
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5neo
RMSD bond length	0.018
RMSD bond angle	2.336
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2219: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.193	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.084	1.750
Rpim	0.029	0.594
Number of reflections	11476	313
<I/σ(I)>	16.4	1.3
Completeness [%]	99.3	100
Redundancy	9.4	9.6
CC(1/2)	0.999	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	280	0.15 M KCl 25% v/v Glycerol 0.05M Bis-Tris 7.0 1.6M Ammonium sulfate