5NOH
HRSV M2-1 core domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.954, 69.241, 46.604 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 46.600 - 1.800 |
| R-factor | 0.1716 |
| Rwork | 0.169 |
| R-free | 0.21030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.814 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.600 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.050 |
| Rpim | 0.033 |
| Number of reflections | 21253 |
| <I/σ(I)> | 12.1 |
| Completeness [%] | 94.8 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M HEPES, pH 7.5 1.4M Tri-sodium citrate |
