5NNV
Structure of a Bacillus subtilis Smc coiled coil middle fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.309, 42.739, 174.601 |
| Unit cell angles | 89.97, 93.64, 89.97 |
Refinement procedure
| Resolution | 50.000 - 3.295 |
| R-factor | 0.2632 |
| Rwork | 0.262 |
| R-free | 0.29830 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.856 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.125 | 3.480 |
| High resolution limit [Å] | 3.295 | 3.295 |
| Rmerge | 0.993 | |
| Number of reflections | 37323 | |
| <I/σ(I)> | 12.33 | 2.8 |
| Completeness [%] | 98.5 | 98.4 |
| Redundancy | 3.6 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 11% PEG3350 50mM Tris pH8 4% MPD 200mM Calcium Acetate |
