5NNT
The dimeric structure of LL-37 crystallized in DPC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.209, 43.703, 54.398 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.558 - 2.209 |
R-factor | 0.264 |
Rwork | 0.263 |
R-free | 0.28730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.740 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.190 |
Rmeas | 0.128 |
Number of reflections | 4568 |
<I/σ(I)> | 8.07 |
Completeness [%] | 98.7 |
Redundancy | 5.9 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 30% PEGMME 3350, 100 mM Tris, ph 8 |