5NNR
Structure of Naa15/Naa10 bound to HypK-THB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978333 |
| Spacegroup name | P 32 |
| Unit cell lengths | 85.348, 85.348, 319.626 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.299 - 3.100 |
| R-factor | 0.2318 |
| Rwork | 0.230 |
| R-free | 0.27170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nnp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.452 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.350 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rpim | 0.065 | 0.977 |
| Number of reflections | 47332 | 4684 |
| <I/σ(I)> | 13.8 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.7 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Mg(NO3)2 20% PEG 3350 protein concentration 25 - 30 mg/ml |
