5NNP

Structure of Naa15/Naa10 bound to HypK-THB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-01
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.872900
Spacegroup name	P 1 21 1
Unit cell lengths	93.319, 106.041, 130.937
Unit cell angles	90.00, 94.98, 90.00

Refinement procedure

Resolution	49.321 - 2.602
R-factor	0.164
Rwork	0.161
R-free	0.21990
RMSD bond length	0.007
RMSD bond angle	0.967
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.321	2.690
High resolution limit [Å]	2.600	2.600
Rpim	0.100
Number of reflections	77910	7407
<I/σ(I)>	8.9
Completeness [%]	99.7	97.3
Redundancy	6.9	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Tris pH 8.5 25 % PEG 3350 protein at 15 mg/ml concentration