EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | JEOL 2100F |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | DIRECT ELECTRON DE-20 (5k x 3k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 10 |
| Number of Particle | 33456 |
| Software | EMAN,RELION,UCSF Chimera |
| Symmetry | D3 |
| CTF correction | PHASE FLIPPING ONLY |
