5NMS

Hsp21 dodecamer, structural model based on cryo-EM and homology modelling

Replaces:  5MB8

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I

EM Data Collection

Microscope	JEOL 2100F
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Detector	DIRECT ELECTRON DE-20 (5k x 3k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	10
Number of Particle	33456
Software	EMAN,RELION,UCSF Chimera
Symmetry	D3
CTF correction	PHASE FLIPPING ONLY