5NMO
Structure of the Bacillus subtilis Smc Joint domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 78.036, 83.865, 64.834 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.294 - 1.899 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.643 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.294 | 1.910 |
| High resolution limit [Å] | 1.890 | 1.810 |
| Rmerge | 0.037 | 1.688 |
| Number of reflections | 65454 | |
| <I/σ(I)> | 16.95 | 1.1 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 4.3 | 8.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 283 | 28% pEG 3350 50 mM Tris pH 8.0 8% MPD 180 mM ammonium acetate |
