5NM9
Crystal structure of the placozoa Trichoplax adhaerens Smad4-MH1 bound to the GGCGC site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976250 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.150, 76.980, 145.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.460 - 2.430 |
| R-factor | 0.222 |
| Rwork | 0.221 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mey |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Outer shell | |
| Low resolution limit [Å] | 2.440 |
| High resolution limit [Å] | 2.430 |
| Number of reflections | 204 |
| <I/σ(I)> | 0.8 |
| Completeness [%] | 100 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 4.8% PEG 4000, 0.1 M sodium acetate pH 4.6 |
