5NLT
CvAA9A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.000, 59.420, 115.450 |
| Unit cell angles | 102.67, 98.89, 89.54 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.20034 |
| Rwork | 0.198 |
| R-free | 0.24441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ach |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.725 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.124 | 0.828 |
| Number of reflections | 90992 | 6493 |
| <I/σ(I)> | 6.7 | 1.22 |
| Completeness [%] | 96.1 | 92.7 |
| Redundancy | 2.16 | 1.69 |
| CC(1/2) | 0.991 | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.6 M (NH4)2SO4 0.1M NaCl 0.1 M HEPES, pH 7.5 |
