5NLP
Auxiliary activity 9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 125.008, 125.008, 125.008 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.200 - 1.590 |
| R-factor | 0.16098 |
| Rwork | 0.160 |
| R-free | 0.17550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ach |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.741 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.200 | 1.700 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmeas | 0.097 | 1.840 |
| Number of reflections | 45264 | 8102 |
| <I/σ(I)> | 17.53 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.51 | 10.82 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 4.1 M NaCl 0.1 M citric acid, pH 4.0 (soaked pH 5.5) |
