5NLH

Chicken GRIFIN (crystallisation pH: 8.5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00
Spacegroup name	P 42 21 2
Unit cell lengths	80.742, 80.742, 39.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.109 - 1.100
R-factor	0.1397
Rwork	0.139
R-free	0.15780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nld
RMSD bond length	0.009
RMSD bond angle	1.439
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.110	1.140
High resolution limit [Å]	1.100	1.100
Number of reflections	52748	4826
<I/σ(I)>	14.33	1
Completeness [%]	99.1	92.12
Redundancy	2	1.9
CC(1/2)		0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	20% Methanol, 0.1M Tris pH 8.5, 0.01M Ca Chloride