5NLG

RRP5 C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9801
Spacegroup name	P 32 2 1
Unit cell lengths	114.010, 114.010, 65.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.476 - 2.350
R-factor	0.2019
Rwork	0.198
R-free	0.24080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.476	39.476	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.093	0.025	0.973
Rmeas	0.104	0.029	1.102
Total number of observations	106545
Number of reflections	20756	269	1491
<I/σ(I)>	13.17	45.94	1.72
Completeness [%]	99.8	97.8	98.6
Redundancy	5.133	4.621	4.446
CC(1/2)	0.998	0.999	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	16-21% PEG1000 100mM Sodium/potassium phosphate, pH 6.0