5NLD

Chicken GRIFIN (crystallisation pH: 7.5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9599
Spacegroup name	P 1 21 1
Unit cell lengths	39.146, 60.736, 53.878
Unit cell angles	90.00, 92.40, 90.00

Refinement procedure

Resolution	39.110 - 0.960
R-factor	0.1374
Rwork	0.137
R-free	0.14940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Theoretical model
RMSD bond length	0.009
RMSD bond angle	1.489
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.110	0.990
High resolution limit [Å]	0.960	0.960
Rmerge	0.023	0.300
Number of reflections	148722	13666
<I/σ(I)>	15.02	2.41
Completeness [%]	97.0	89.78
Redundancy	1.9	1.7
CC(1/2)		0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	5% w/v 2-Propanol, 0.1M HEPES pH 7.5