5NLC
Structure of PipY, the COG0325 family member of Synechococcus elongatus PCC7942,without PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.618, 41.778, 77.761 |
| Unit cell angles | 90.00, 108.15, 90.00 |
Refinement procedure
| Resolution | 44.610 - 1.900 |
| R-factor | 0.15963 |
| Rwork | 0.157 |
| R-free | 0.20744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w8g |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.835 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.610 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.045 | |
| Rpim | 0.023 | 0.170 |
| Number of reflections | 32801 | 2072 |
| <I/σ(I)> | 20.6 | 4 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 4.6 | 3.8 |
| CC(1/2) | 0.998 | 0.935 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.1 M Tris-HCl, pH 8.5 0.2 M Magnesium chloride 25% (wt/vol) Polyethylene glycol 3350 |
