5NLA

Crystal structure of the AraC-like transcriptional activator CuxR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978
Spacegroup name	P 43 2 2
Unit cell lengths	80.662, 80.662, 125.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.660 - 2.700
R-factor	0.23808
Rwork	0.236
R-free	0.28101
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.383
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.660	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.055
Number of reflections	11906	1134
<I/σ(I)>	23.7	2.2
Completeness [%]	99.7	98
Redundancy	8.3
CC(1/2)	1.000	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	293	1.2M NaCl, 0.1 M Na-acetate pH 4.8