5NL9
Crystal structure of a peroxide stress regulator from Leptospira interrogans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2013-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.28215 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.683, 56.100, 68.794 |
| Unit cell angles | 90.00, 105.82, 90.00 |
Refinement procedure
| Resolution | 46.840 - 1.900 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.840 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | 0.852 |
| Number of reflections | 27475 | |
| <I/σ(I)> | 13.9 | 2.6 |
| Completeness [%] | 97.3 | 95.3 |
| Redundancy | 6.3 | 6.2 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 301 | 20% (w/v) PEG-3350, 0.2M potassium sodium tartrate |
