5NL8

Pex4 of Hansenula Polymorpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03321
Spacegroup name	P 41 21 2
Unit cell lengths	46.350, 46.350, 206.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.220 - 2.200
R-factor	0.2119
Rwork	0.209
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y9m
RMSD bond length	0.004
RMSD bond angle	0.796
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.220	2.093
High resolution limit [Å]	2.020	2.020
Rmerge	0.147	1.014
Number of reflections	14409	1206
<I/σ(I)>	6.21	0.98
Completeness [%]	92.0	80
Redundancy	4.13	1.63
CC(1/2)	0.995	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M MES pH 6.5 50% (v/v) PEG-200