5NKX
HRSV M2-1 core domain, P3221 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-12-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03321 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 91.949, 91.949, 97.799 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.974 - 2.000 |
| R-factor | 0.191709571525 |
| Rwork | 0.190 |
| R-free | 0.22898 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c3e |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.533 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 8.970 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.052 | |
| Rmeas | 0.073 | |
| Number of reflections | 52178 | |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 97.6 | |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 2.4M SODIUM MALONATE |
