5NKM
SMG8-SMG9 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 111.085, 111.085, 374.474 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 62.412 - 2.493 |
R-factor | 0.2264 |
Rwork | 0.225 |
R-free | 0.26020 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.568 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXD |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.360 | 2.583 |
High resolution limit [Å] | 2.493 | 2.493 |
Rmerge | 0.137 | 2.587 |
Number of reflections | 188934 | |
<I/σ(I)> | 27.25 | |
Completeness [%] | 98.0 | |
Redundancy | 39 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 285 | 10% PEG3350, 0.1M Tris pH 8.5 |