5NJK
PTB domain of human Numb isoform-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-27 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.270, 81.870, 235.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.668 - 3.130 |
R-factor | 0.2312 |
Rwork | 0.230 |
R-free | 0.26040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fow |
RMSD bond length | 0.015 |
RMSD bond angle | 1.857 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.240 |
High resolution limit [Å] | 3.130 | 3.130 |
Rmerge | 0.156 | 0.993 |
Number of reflections | 26321 | |
<I/σ(I)> | 9.5 | 1.6 |
Completeness [%] | 99.4 | 98.1 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 25 % PEG 3350, 0.2 M Lithium Sulfate, and 0.1 M Bis-Tris pH 6 |