5NJK
PTB domain of human Numb isoform-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-27 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.270, 81.870, 235.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.668 - 3.130 |
| R-factor | 0.2312 |
| Rwork | 0.230 |
| R-free | 0.26040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fow |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.857 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.240 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Rmerge | 0.156 | 0.993 |
| Number of reflections | 26321 | |
| <I/σ(I)> | 9.5 | 1.6 |
| Completeness [%] | 99.4 | 98.1 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 25 % PEG 3350, 0.2 M Lithium Sulfate, and 0.1 M Bis-Tris pH 6 |
