5NJJ
PTB domain of human Numb isoform-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.999 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 113.770, 120.440, 237.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.573 - 2.700 |
| R-factor | 0.2703 |
| Rwork | 0.269 |
| R-free | 0.30330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fow |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.872 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.097 | 0.130 |
| Number of reflections | 22466 | |
| <I/σ(I)> | 18.4 | 2.2 |
| Completeness [%] | 99.6 | 98.6 |
| Redundancy | 13.2 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 25 % PEG 3350, 0.2 M Lithium Sulfate, and 0.1 M Bis-Tris pH 6 |
