5NJ7
The X-ray structure of the adduct formed in the reaction between bovine pancreatic ribonuclease and arsenoplatin-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-09 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.535, 32.855, 72.939 |
Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
Resolution | 72.940 - 2.150 |
R-factor | 0.1904 |
Rwork | 0.187 |
R-free | 0.25049 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jvt |
RMSD bond length | 0.014 |
RMSD bond angle | 1.709 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.940 | 2.150 |
High resolution limit [Å] | 2.150 | |
Rmerge | 0.117 | 0.504 |
Number of reflections | 13274 | |
<I/σ(I)> | 10 | 2.9 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 298 | 22% w/v PEG4K, 10 mM sodium acetate pH 5.1. Crystals of RNase A-AP-1 were formed by soaking pre-grown RNase A crystals for 3 h in a solution containing 5 mM AP-1 dissolved in DMSO and then added to the reservoir. |