5NIW
Glucose oxydase mutant A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 128.100, 128.100, 77.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 110.940 - 1.800 |
| R-factor | 0.15583 |
| Rwork | 0.154 |
| R-free | 0.19089 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.206 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.930 |
| High resolution limit [Å] | 1.790 |
| Number of reflections | 67817 |
| <I/σ(I)> | 12.1 |
| Completeness [%] | 98.3 |
| Redundancy | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 280 | 100 mM HEPES 45% v/v dioxane |
