5NIR
Crystal structure of collagen 2A vWC domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.930, 60.160, 86.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.370 - 1.740 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | AutoPROC |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.370 | 1.750 |
| High resolution limit [Å] | 1.740 | 1.744 |
| Rmerge | 0.042 | 0.709 |
| Number of reflections | 17667 | |
| <I/σ(I)> | 22.7 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES, pH 7.5, 40% PEG 200 |
