5NHK
Structure of Ferric uptake regulator from francisella tularensis with Iron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.350, 90.040, 64.050 |
| Unit cell angles | 90.00, 93.53, 90.00 |
Refinement procedure
| Resolution | 42.214 - 1.800 |
| R-factor | 0.2052 |
| Rwork | 0.204 |
| R-free | 0.24310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nbc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.874 |
| Data scaling software | XSCALE (20151231) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.210 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 55503 |
| <I/σ(I)> | 9.18 |
| Completeness [%] | 99.2 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50mM MES pH 5.8, 20% W/V PEG 3350, 200mM Magnesium chlorides hexahydrate and 10mM of (NH4)2Fe(SO4)2. 6 H2O in anaerobic condition |
