5NGL
The endo-beta1,6-glucanase BT3312
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.7749 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.379, 78.766, 145.526 |
| Unit cell angles | 90.00, 94.94, 90.00 |
Refinement procedure
| Resolution | 48.790 - 1.850 |
| R-factor | 0.19033 |
| Rwork | 0.188 |
| R-free | 0.22797 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ril |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.521 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.790 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 116665 | 5762 |
| <I/σ(I)> | 7.8 | 1.6 |
| Completeness [%] | 99.2 | 86.2 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.996 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM Potassium thiocyanate 20% PEG 3350 |
