5NGK
The endo-beta1,6-glucanase BT3312
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.418, 156.001, 78.050 |
| Unit cell angles | 90.00, 100.41, 90.00 |
Refinement procedure
| Resolution | 48.240 - 1.900 |
| R-factor | 0.18379 |
| Rwork | 0.182 |
| R-free | 0.22124 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ril |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.526 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.240 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 114209 | 4871 |
| <I/σ(I)> | 9 | 1.8 |
| Completeness [%] | 97.4 | 97.6 |
| Redundancy | 3.8 | 4 |
| CC(1/2) | 0.996 | 0.466 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM Sodium Bromide 100 mM Bis Tris Propane pH 6.5 and 20 % PEG 3350. |
