5NFI
The fimbrial anchor protein Mfa2 from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97239 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.600, 83.210, 38.740 |
| Unit cell angles | 90.00, 94.76, 90.00 |
Refinement procedure
| Resolution | 41.605 - 2.508 |
| R-factor | 0.1997 |
| Rwork | 0.197 |
| R-free | 0.26260 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.218 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.605 | 2.610 |
| High resolution limit [Å] | 2.508 | 2.508 |
| Rmerge | 0.130 | 0.832 |
| Rpim | 0.033 | 0.408 |
| Number of reflections | 11043 | 1236 |
| <I/σ(I)> | 14.7 | 1.7 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 12.7 | 4.9 |
| CC(1/2) | 0.998 | 0.664 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Bis-Tris propane pH 7.0, 20%, PEG 3350 and 0.2 M sodium iodide |
