5NFF

Crystal structure of GP1 receptor binding domain from Morogoro virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9194
Spacegroup name	P 32
Unit cell lengths	127.769, 127.769, 251.704
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.188 - 2.615
R-factor	0.1757
Rwork	0.174
R-free	0.20740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zjf
RMSD bond length	0.009
RMSD bond angle	1.180
Data reduction software	XDS (November 3, 2014)
Data scaling software	XSCALE (November 3, 2014)
Phasing software	PHASER
Refinement software	PHENIX (dev_2236)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.190	2.700
High resolution limit [Å]	2.610	2.610
Rmeas	0.149	0.963
Number of reflections	138414
<I/σ(I)>	9.09	1.58
Completeness [%]	98.9	92.2
Redundancy	4	3.8
CC(1/2)	0.987	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	26% PEG 8000, 100 mM sodium citrate pH 4.0 and 0.01% octylphenoxypolyethoxyethanol (IGEPAL)