5NF8
Solution structure of detergent-solubilized Rcf1, a yeast mitochondrial inner membrane protein involved in respiratory Complex III/IV supercomplex formation
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE III | 900 | |
| 2 | Bruker | AVANCE III | 700 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 1H-15N HSQC |
| 10 | 3 | 3 | 3D HCCH-TOCSY |
| 11 | 3 | 3 | 3D CC(CO)NH |
| 12 | 3 | 3 | 3D H(CCO)NH |
| 13 | 3 | 3 | 2D 1H-13C HSQC |
| 14 | 3 | 3 | 3D N-NOESY-HSQC |
| 15 | 3 | 3 | 3D C-NOESY-HSQC |
| 16 | 4 | 4 | 2D 1H-13C HSQC |
| 17 | 4 | 4 | 3D N-NOESY-HSQC |
| 18 | 4 | 4 | 3D C-NOESY-HSQC |
| 19 | 5 | 5 | 2D 1H-13C HSQC |
| 2 | 1 | 1 | 2D 1H-15N HSQC |
| 20 | 5 | 5 | 3D C-NOESY-HSQC |
| 21 | 6 | 6 | 3D F1-13C/15N-filtered, F3-15N-edited-NOESY-HSQC |
| 22 | 6 | 6 | 3D F1-13C/15N-filtered, F3-13C-edited-NOESY-HSQC |
| 3 | 2 | 2 | 3D HNCACB |
| 4 | 2 | 2 | 3D HN(COCA)CB |
| 5 | 2 | 2 | 3D HNCO |
| 6 | 2 | 2 | 3D HN(CA)CO |
| 7 | 2 | 2 | 3D HNCA |
| 8 | 2 | 2 | 3D HN(CO)CA |
| 9 | 2 | 2 | 3D N-NOESY-HSQC |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 5 | 1 | 323 |
| 2 | 5 | 1 | 323 |
| 3 | 5 | 1 | 323 |
| 4 | 5 | 1 | 323 |
| 5 | 5 | 1 | 323 |
| 6 | 5 | 1 | 323 |
Conformers
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 15 |
