5NEX
The structure of the G. violaceus guanidine II riboswitch P1 stem-loop with agmatine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9179 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 55.639, 55.639, 135.927 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.416 - 1.720 |
| R-factor | 0.2022 |
| Rwork | 0.201 |
| R-free | 0.23310 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.383 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2219: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.420 | 1.750 |
| High resolution limit [Å] | 1.719 | 1.719 |
| Rmerge | 0.129 | 1.500 |
| Rpim | 0.031 | 0.948 |
| Number of reflections | 16643 | 452 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 18.5 | 17.8 |
| CC(1/2) | 0.996 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.01 M Magnesium Acetate, 0.05 M MES pH 5.6, 2.5 M Ammonium Sulfate soaking with 500mM agmatine |
