5NEP
The structure of the G. violaceus guanidine II riboswitch P1 stem-loop with methylguanidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 55.500, 55.500, 132.379 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.750 - 1.600 |
| R-factor | 0.2072 |
| Rwork | 0.207 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.466 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2219: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.750 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.062 | 1.500 |
| Rpim | 0.015 | 0.726 |
| Number of reflections | 19703 | 529 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 100.0 | 99.1 |
| Redundancy | 18.83 | 19.1 |
| CC(1/2) | 0.999 | 0.916 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.01 M Magnesium Acetate, 0.05 M MES pH 5.6, 2.5 M Ammonium Sulfate Soaking with 10mM Methylguanidine |
