5NEN
Crystal structure of the soluble domain of LipC, a membrane fusion protein of a type I secretion system
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2016-11-11 |
Detector | BRUKER SMART 6500 |
Wavelength(s) | 1 |
Spacegroup name | P 32 |
Unit cell lengths | 126.314, 126.314, 71.405 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.346 - 2.901 |
R-factor | 0.2542 |
Rwork | 0.252 |
R-free | 0.30070 |
Structure solution method | SIRAS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.489 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 28216 |
<I/σ(I)> | 28.1 |
Completeness [%] | 100.0 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 32% ethylene glycol |