5NEF
The structure of the G. violaceus guanidine II riboswitch P1 stem-loop with guanidine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9201 |
Spacegroup name | H 3 2 |
Unit cell lengths | 55.700, 55.700, 132.560 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.187 - 1.910 |
R-factor | 0.2054 |
Rwork | 0.203 |
R-free | 0.25790 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.877 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2219: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.190 | 1.940 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.085 | 1.500 |
Rpim | 0.030 | 0.725 |
Number of reflections | 11824 | 313 |
<I/σ(I)> | 16.1 | |
Completeness [%] | 99.3 | 100 |
Redundancy | 9.4 | 9.6 |
CC(1/2) | 0.999 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 280 | 0.15 M KCl 25% v/v Glycerol 0.05M Bis-Tris 7.0 1.6M Ammonium sulfate |