5NE4
Crystal Structure of Foot and Mouth Disease Virus O PanAsia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 294 |
Detector technology | PIXEL |
Collection date | 2014-02-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9778 |
Spacegroup name | I 2 3 |
Unit cell lengths | 345.010, 345.010, 345.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.210 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bbt |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (v1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.270 | 0.880 |
Number of reflections | 229892 | |
<I/σ(I)> | 3.1 | |
Completeness [%] | 76.9 | 45.9 |
Redundancy | 2.1 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 1.5 M ammonium sulfate, 100 mM bis-Tris propane, pH 7.0. |