5NE0

Room temperature in-situ structure of hen egg-white lysozyme from crystals enclosed between ultrathin silicon nitride membranes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2017-02-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	79.500, 79.500, 37.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.750 - 1.570
R-factor	0.1665
Rwork	0.166
R-free	0.18390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lyz
RMSD bond length	0.005
RMSD bond angle	0.770
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.750	1.610
High resolution limit [Å]	1.570	1.570
Rmeas	0.050	0.847
Number of reflections	30841	1669
<I/σ(I)>	13.74	1.04
Completeness [%]	95.4	95.2
Redundancy	3.96
CC(1/2)	0.990	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	90 ul (50mg/ml H2O) lysozyme solution + 20 ul of the precipitant solution (0.1M NaAc 0.6M NaCl) and 10ul 25% ethylene glycol (in water) were mixed together. 20 microliters from this mixture deposited onto the silicon nitride chip.