5NDY

crystal structure of variants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-16
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	142.539, 147.662, 90.347
Unit cell angles	90.00, 108.99, 90.00

Refinement procedure

Resolution	49.773 - 1.949
R-factor	0.2204
Rwork	0.218
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.918
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2119: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.773
High resolution limit [Å]	1.949
Rmerge	0.069	1.068
Rmeas	0.075	0.446
Rpim	0.028	1.160
Number of reflections	125165
<I/σ(I)>	17.16
Completeness [%]	97.4
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	PEG, Tris Cl