5NDG
Crystal structure of geneticin (G418) bound to the yeast 80S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2014-11-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 443.590, 297.320, 300.150 |
| Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
| Resolution | 91.755 - 3.700 |
| R-factor | 0.2105 |
| Rwork | 0.210 |
| R-free | 0.25300 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.447 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 91.755 | 115.000 | 3.800 |
| High resolution limit [Å] | 3.700 | 100.000 | 3.700 |
| Rmerge | 0.159 | 12.300 | 1.697 |
| Rmeas | 0.176 | 17.395 | 1.892 |
| Number of reflections | 814796 | 1 | 62491 |
| <I/σ(I)> | 8.28 | 0.9 | |
| Completeness [%] | 99.9 | 7.1 | 99.9 |
| Redundancy | 5.209 | 2 | 5.034 |
| CC(1/2) | 0.998 | 0.478 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine |
