5ND6
Crystal structure of apo transketolase from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.912 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.211, 74.311, 133.723 |
| Unit cell angles | 90.00, 119.30, 90.00 |
Refinement procedure
| Resolution | 116.600 - 1.580 |
| R-factor | 0.16097 |
| Rwork | 0.159 |
| R-free | 0.19225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1itz |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.967 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.600 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.049 | 0.567 |
| Number of reflections | 193102 | |
| <I/σ(I)> | 12.7 | 1.9 |
| Completeness [%] | 98.1 | 97.9 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10 % w/v PEG 6K, 5 % v/v MPD and 0.1 M MES pH 6.5-7.0 or 0.1 M HEPES pH 7.0-8.0. |
