5ND5
Crystal structure of transketolase from Chlamydomonas reinhardtii in complex with TPP and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.489, 75.930, 103.759 |
| Unit cell angles | 90.00, 109.99, 90.00 |
Refinement procedure
| Resolution | 97.510 - 1.740 |
| R-factor | 0.14715 |
| Rwork | 0.145 |
| R-free | 0.18603 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1itz |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.939 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.520 | 1.820 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.053 | 0.240 |
| Number of reflections | 114961 | |
| <I/σ(I)> | 11.9 | 1.7 |
| Completeness [%] | 76.2 | 64.6 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10 % w/v PEG 6K, 5 % v/v MPD and 0.1 M MES pH 6.5-7.0 or 0.1 M HEPES pH 7.0-8.0 |
