5NCK

The Crystal Structure of N-acetylmannosamine kinase in Fusobacterium nucleatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-29
Detector	MAR CCD 130 mm
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	126.520, 126.520, 108.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.936 - 2.230
R-factor	0.1788
Rwork	0.177
R-free	0.21960
RMSD bond length	0.010
RMSD bond angle	1.089
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	54.785
High resolution limit [Å]	2.150
Number of reflections	54608
<I/σ(I)>	0.8
Completeness [%]	99.0
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	30% PEG 4000,0.2 M lithium sulfate monohydrate, 0.1 M tris hydrochloride